Coding

Part:BBa_K4392012:Design

Designed by: Atharva Valanju   Group: iGEM22_IISER-Pune-India   (2022-10-11)


exoC gene from Azospirillum brasilense


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 164
    Illegal NgoMIV site found at 295
    Illegal NgoMIV site found at 377
    Illegal NgoMIV site found at 529
    Illegal NgoMIV site found at 961
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 810


Design Notes

FP: TATATCTAGAATGAGCGAAGCCCACACTTT (Tm=61.3 C; %GC=40) RP: TATATCTAGATCAGTGGAGCTGCCGCCGTC (Tm=65.9 C; %GC=53)



Source

Derived from the genome of A. brasilense

References